desK [2017-8-11 16:0:3]

two-component sensor kinase, regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

locus: BSU19190

pI: 9.43

mw: 42.51 kDa

protein length: 370 aa

gene length: 1110 bp

function: regulation of cold shock expression of [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

product: two-component sensor kinase

essential: no

synonyms: yocF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 2,090,574 → 2,091,686

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR]

[SW|Domains]

• 5 transmembrane helices
• cytoplasmatic C-terminal trail

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• unsaturated fatty acids are negative effectors of the system

Structure

• [PDB|3EHF] [Pubmed|19805278]
• [PDB|5IUJ]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphotransfer state with low Mg2+ [20 mM]) [Pubmed|27938660]
• [PDB|5IUK]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphotransfer state with high Mg2+ [150 mM] and BeF3) [Pubmed|27938660]
• [PDB|5IUM](phosphorylated wild type [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]C) [Pubmed|27938660]
• [PDB|5IUN]([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK]-[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR] complex in the phosphatase state) [Pubmed|27938660]

[SW|Localization]

• membrane (transmembrane segments)

Additional information

• [protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK] has the ability to sense changes in membrane fluidity [Pubmed|17087771]

Expression and Regulation

Operons

• genes: [gene|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK]-[gene|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]
  description: [Pubmed|11285232]

  regulation

  • induced by cold shock (12-fold) [Pubmed|12399512]

Biological materials

Mutant

• MGNA-A327 (yocF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/327 NBRP B. subtilis, Japan]

Labs working on this gene/protein

• [SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
• [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 20117042,17087771,24819366

Original publications

• 10094672,14734164,12399512,20507988,19233289,11285232,19805278,15090506,12207704,20705470,23356219,24574048,24522108,25406381,26172072,27528507,27938660,28475313